The HuGoDeCA data portal gathers datasets about Human Gonad Developmental Cells, resulting either from single cell analysis (scRNAseq / scATACseq), Spatial Transcriptomics (ST), In Situ Sequencing data (ISS) or 3D cleared-sample imaging.
This dataset aims at identifying all major gonadal cell types at distinct differentiation stages, including for instance pre-Sertoli cells at the onset of SRY expression. It extends existing data to broader developmental stages to include bipotential gonads before sex determination with more differentiated ones from 2nd semester embryos
This dataset results from the scRNA-seq analysis performed to understand how cell lineage differentiation is affected by the manipulation of the DHH signaling pathway. The manipulation has been done with known receptor agonists (e.g. SAG) and inhibitors (e.g. cyclopamine) of the different steps of this pathway on organotypic cultures of 7 PCW testes.